Analysis of Amino Acids (Amino Acid Profiling) by HPLC

Analysis of amino acids by High-Performance Liquid Chromatography (HPLC) is a laboratory technique used to identify and quantify the different amino acids in a sample. It involves separating the amino acids in a mixture based on their chemical properties and measuring their concentrations. In this process, a liquid sample containing amino acids is injected into an HPLC system, where it is pushed through a column filled with a special material that separates the amino acids as they pass through. By detecting and measuring the time it takes for each amino acid to elute from the column, scientists can determine the type and quantity of amino acids present in the sample. This method is crucial for protein analysis, nutritional studies, and medical diagnostics, providing valuable information about the composition of proteins and amino acid imbalances in various biological and clinical samples.

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